Menu

01
02
03
04
05
06
07
08
01
02
03
04
05
06
07
08
Oileia logo
Oileia logo

Selection of parents

The following steps are followed:

  1. Prospecting among the various existing olive varieties.
  2. Selection of the best genotypes to intervene in the cross.
  3. Selection of mother and father olive trees, based on remarkable phenotypic and genotypic criteria (molecular markers).

Pollen collection

It is done as follows:

  1. By gradually shaking the branches of the selected olive trees, in a non-destructive way.
  2. Pollen is dropped in a directed manner to facilitate handling.
  3. The most suitable times for collection are chosen, avoiding days with high relative humidity or high wind, and considering the flowering time of each variety.

Pollen is kept below 0°C and homogenized with inert material until use.

Controlled pollination

It contemplates the following actions:

  1. In the mother trees, the most convenient branches are identified for subsequent pollination, controlling their inclination, number of flower clusters, quality of the branch, etc.
  2. The female flowers are isolated with cellulose bags just before the start of the opening, to avoid possible pollen contamination by flying pollen from other varieties.
  3. Once the pollination has been carried out, the bags are closed again to avoid possible contamination by another type of pollen.
  4. This process is carried out several times to ensure the fertilization of as many flowers as possible.
  5. Once it is verified that the flowering has finished, the bags are carefully removed so that the fruit ripens well.
  6. Branches where controlled pollination has been carried out are labelled for subsequent monitoring.

Obtaining seeds

It is done as follows:

  1. Manually, indicating the origin of each crossing and maintaining its traceability.
  2. From 23 to 560 seeds are collected depending on the variety.
  3. Seeds with good physical appearance are selected.
  4. They are prepared slowly and carefully.
  5. The embryos are extracted to achieve the highest possible germination percentage.

Stratification

Why do it?

  1. The embryo of the olive fruit is protected by a very hard endocarp (bone).
  2. The endocarp constitutes a mechanical barrier that hinders the germination of some seeds.
  3. Seed stratification is a procedure that aims to facilitate germination, increasing success rates.

How do we do it?

  1. Different methods are applied, with a high level of precision.
  2. In this case, they were stratified in camera under controlled dark, temperature and humidity conditions.
  3. The humidity of the substrate of the trays was monitored to know its evolution, and thus by means of water contributions, to maintain the humidity at optimum levels.

Germination

  • Thirty days from the beginning of the stratification, the radicles and cotyledons emerged in the alveolar trays.
  • When the plants had approximately 3 pairs of leaves, they were changed to higher volume pots with the same substrate used in the alveoli and transferred to a controlled environment chamber.
  • In this camera the photoperiod, the light, the humidity of the substrate and the environment, and the temperature are regulated.

Seedling development

  • The favorable conditions in the controlled environment chambers allowed an optimal development of the plants.
  • Reaching heights of up to 40 cm after 4 months from germination.
  • From a height of about 30 cm, tutors were placed on them.
  • In June, after 5 months in controlled conditions, the plants move abroad, where acclimatization to more adverse environmental conditions is carried out gradually.
  • The plants are first placed under shading mesh, which is gradually removed.

Agronomic selection

In theory, any agronomic character is likely to be improved depending on:

  • The threshold applied.
  • Limitations established by other characters.
  • That the character we are pursuing has been inherited from the selected parents.

In Oileia, the possibilities of finding the ideal genotype (greater resistance to cold, early maturing and with oil with a high content of polyphenols) are increased thanks to the fact that:

  • We have expert geneticists with great experience in this branch.
  • State-of-the-art facilities and tools, so that you can practice selection on both morphological and phenological characteristics as well as genetic, biochemical and metabolic characteristics.
  • Have about 3,000 different genotypes from 12 varieties selected for their agronomic behavior.

R&D

Biologia verde

Genetic
improvement

Microscope

Biotechnology and Molecular Biology

Planta

Plantation management

Among the objectives of a genetic improvement program are:
  • Improve existing genetic material, even for a single character.
  • Vary, improve or select characteristics such as: adaptability to adverse conditions, tolerance to pests and diseases, productivity, polyphenolic content, etc.
Oleia is developing a genetic improvement program in collaboration with the University of Córdoba that includes a large team of geneticists and biotechnologists with the aim of:
  • Obtaining new varieties of olive with greater resistance to cold, early maturing and producing an oil with a high content of polyphenols.
The use of biotechnology and molecular biology, fundamental tools used by Oileia allows us to develop different activities such as:
  • Obtaining new varieties, a long-lasting and complex process,especially in woody species, involving a large number of factors.
  • Accelerate the production of plants of the new selected genotypes, by in vitro culture.
  • In vitro evaluation of different parameters, such as tolerance to biotic factors (pests and diseases) or abiotic factors (salinity, drought, extreme temperatures).
  • Characterize our varieties by using DNA markers, associate markers with some phenotypic characteristics, certify the varietal guarantee of our plants, etc.
  • Molecular diagnosis to assess the health status of all our plant material.
Oileia maximizes the profitability, economic and environmental sustainability of the plantations, paying attention to different agronomic lines such as:
  • Driving system.
  • Deficit irrigation.
  • Ecological management.
  • Directed mycorrhization.
  • Precision fertilization.
  • Phytosanitary and weed control.
Selection of parents

The following steps are followed:

  1. Prospecting among the various existing olive varieties.
  2. Selection of the best genotypes to intervene in the cross.
  3. Selection of mother and father olive trees, based on remarkable phenotypic and genotypic criteria (molecular markers).
01
Pollen collection

It is done as follows:

  1. By gradually shaking the branches of the selected olive trees, in a non-destructive way.
  2. Pollen is dropped in a directed manner to facilitate handling.
  3. The most suitable times for collection are chosen, avoiding days with high relative humidity or high wind, and considering the flowering time of each variety.
  4. Pollen is kept below 0°C and homogenized with inert material until use.
02
Controlled pollination

It contemplates the following actions:

  1. In the mother trees, the most convenient branches are identified for subsequent pollination, controlling their inclination, number of flower clusters, quality of the branch, etc.
  2. The female flowers are isolated with cellulose bags just before the start of the opening, to avoid possible pollen contamination by flying pollen from other varieties.
  3. Once the pollination has been carried out, the bags are closed again to avoid possible contamination by another type of pollen.
  4. This process is carried out several times to ensure the fertilization of as many flowers as possible.
  5. Once it is verified that the flowering has finished, the bags are carefully removed so that the fruit ripens well.
  6. Branches where controlled pollination has been carried out are labelled for subsequent monitoring.
03
Obtaining seeds

It is done as follows:

  1. Manually, indicating the origin of each crossing and maintaining its traceability.
  2. From 23 to 560 seeds are collected depending on the variety.
  3. Seeds with good physical appearance are selected.
  4. They are prepared slowly and carefully.
  5. The embryos are extracted to achieve the highest possible germination.
04
Stratification

Why do it?

  1. The embryo of the olive fruit is protected by a very hard endocarp (bone).
  2. The endocarp constitutes a mechanical barrier that hinders the germination of some seeds.
  3. Seed stratification is a procedure that aims to facilitate germination, increasing success rates.

How do we do it?

  1. Different methods are applied, with a high level of precision.
  2. In this case, they were stratified in camera under controlled dark, temperature and humidity conditions.
  3. The humidity of the substrate of the trays was monitored to know its evolution, and thus by means of water contributions, to maintain the humidity at optimum levels.
05
Germination
  • Thirty days from the beginning of the stratification, the radicles and cotyledons emerged in the alveolar trays.
  • When the plants had approximately 3 pairs of leaves, they were changed to higher volume pots with the same substrate used in the alveoli and transferred to a controlled environment chamber.
  • In this camera the photoperiod, the light, the humidity of the substrate and the environment, and the temperature are regulated.
06
Seedling development
  • The favorable conditions in the controlled environment chambers allowed an optimal development of the plants.
  • Reaching heights of up to 40 cm after 4 months from germination.
  • From a height of about 30 cm, tutors were placed on them.
  • In June, after 5 months in controlled conditions, the plants move abroad, where acclimatization to more adverse environmental conditions is carried out gradually.
  • The plants are first placed under shading mesh, which is gradually removed.
07
Agronomic selection

In theory, any agronomic character is likely to be improved depending on:

  • The threshold applied.
  • Limitations established by other characters.
  • That the character we are pursuing has been inherited from the selected parents.

In Oileia, the possibilities of finding the ideal genotype (greater resistance to cold, early maturing and with oil with a high content of polyphenols) are increased thanks to the fact that:

  • We have expert geneticists with great experience in this branch.
  • State-of-the-art facilities and tools, so that you can practice selection on both morphological and phenological characteristics as well as genetic, biochemical and metabolic characteristics.
  • Have about 3,000 different genotypes from 12 varieties selected for their agronomic behavior.
08

Contacto

Copyright © 2020 Oileia

Whatsapp
Oileia
Customer service

Hi..

How can we help you?

Oileia
Send